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[Analysis Case] Characterization of Culture Medium

[Analysis Case] Characterization of Culture Medium

For the identification of main components, we conduct semi-quantitative analysis of multiple components, and for the management of specific components, we perform quantitative analysis, tailoring the analysis to the intended use.

The culture medium contains various compounds, including amino acids, organic acids, vitamins, nucleic acid-related compounds, and metabolites secreted from cells. At Kaneka Techno Research, we can semi-quantitatively and quantitatively analyze many organic and inorganic components contained in the culture medium using various analytical methods. By performing multivariate analysis on the obtained data, we can identify the component factors that influence production rates and provide useful information for problem-solving. ■ Analytical Methods Identification of main components: Semi-quantitative analysis of multiple components Management of target components: Quantitative analysis, etc. 〈Equipment Used〉 LC-MS/MS, CE-TOF/MS, GC/MS, IC, ICP-AES, ICP-MS, etc. This technology can also be applied to research and development in the medical field, such as regenerative cells, and the food industry.

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[Analysis Case] Combustion Gas and Heating Generation Gas Analysis

[Analysis Case] Combustion Gas and Heating Generation Gas Analysis

We will propose the appropriate analysis method according to your objectives!!

◎Heating Emission Gas Analysis Qualitative and quantitative analysis of gases generated by heating will be conducted. Materials and products may emit gases during their manufacturing processes due to heating, and these gases can potentially have adverse effects on other raw materials or products. It is possible to analyze the gases generated under conditions equivalent to the heating conditions in the manufacturing process of the sample. - Heating Method Carrier gas (nitrogen, argon, air, etc.) is flowed through a combustion tube at a specified flow rate while heating to the set temperature. *Depending on the customer's objectives, analysis can be performed under various conditions, including different types of carrier gases (nitrogen, argon), flow rates, temperatures, etc. - Collection and Analysis Method Combustion gases will be collected and analyzed using a collection method suitable for the target gases. ◎Combustion Gas Analysis Qualitative and quantitative analysis of gas components generated during combustion under the conditions specified in "Analysis Method for Plastic Combustion Gases" JIS K 7217 will be conducted. (Compliant with JIS K 7217 Method A) By analyzing combustion gases, the safety and environmental impact of materials can be evaluated.

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[Analysis Case] Characterization of Glycosylated Proteins (LC-TOFMS)

[Analysis Case] Characterization of Glycosylated Proteins (LC-TOFMS)

It is possible to efficiently advance the development and quality assurance of biopharmaceuticals!

The LC-TOFMS (Liquid Chromatography Time-of-Flight Mass Spectrometry) method is useful for the characterization analysis of proteins (such as molecular weight analysis, peptide mapping, and glycan analysis). We will introduce a case study of the analysis and examination of the glycan (glycoform and glycan attachment sites) of IgG antibodies using the LC-TOFMS method. ■Analysis Sample: IgG Antibody ■Analysis Method: LC-TOFMS ■Analysis Results - Glycoform Analysis By analyzing the molecular weight, it is possible to estimate the glycoforms of glycoproteins. LC-TOFMS measurement of the IgG antibody revealed the presence of five different glycoforms. - Glycan Attachment Site Analysis The IgG antibody was fragmented through enzymatic treatment and measured by LC-TOFMS. The analysis of the measurement results using dedicated software showed that a N-linked glycan (G0F, G1F, G2F) was attached to the asparagine of the peptide fragment with the amino acid sequence EEQFNSTFR. *For detailed results, please refer to the PDF document.

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Announcement of Exhibition Participation "JASIS 2023"

Announcement of Exhibition Participation "JASIS 2023"

We will be exhibiting at JASIS2023!!

We will be exhibiting at our booth at "JASIS2023," which will be held at Makuhari Messe from September 6 to 8, 2023. 【Exhibition Details】 ◆ Analysis of lithium-ion battery anode under non-atmospheric exposure ◆ Analysis of gases generated from heating and combustion ◆ Analysis of amino acid dipeptides Exhibition Location: Mini/Solution Exhibition Corner (S-9)

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[Analysis Case] Analysis of Nitrosamines (LC-MS/MS Analysis)

[Analysis Case] Analysis of Nitrosamines (LC-MS/MS Analysis)

This is an example of conducting LC-MS/MS analysis (liquid chromatography-tandem mass spectrometry) of nine types of nitrosamines standard samples.

In recent years, N-nitrosodimethylamine, a carcinogenic substance, has been detected in sartan-based pharmaceuticals. According to the Ministry of Health, Labour and Welfare's notification titled "Self-Inspection Regarding the Risk of Nitrosamine Contamination in Pharmaceuticals (dated October 8, 2021)," nitrosamines in pharmaceuticals must be below specified limits, which requires the measurement of trace amounts of the relevant components. This document introduces a case of LC-MS/MS analysis (liquid chromatography-tandem mass spectrometry) of standard samples of nine types of nitrosamines mentioned in the notification. ■ Analysis Samples - Standard samples of nine types of nitrosamines ■ Analysis Conditions - Equipment: AB SCIEX SCIEX QTRAP 5500 SYSTEM - Ionization Method: ESI positive ion mode - Measurement Mode: MRM ■ Analysis Results For each nitrosamine, peaks at a concentration of 1 ng/mL, necessary for limit value evaluation, can be detected.

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[Analysis Case] Quantitative Analysis of CO2 Emitted During Cement Heating

[Analysis Case] Quantitative Analysis of CO2 Emitted During Cement Heating

One method to understand CO2 emissions is the TG-MS (Thermogravimetric Mass Spectrometry) method, which allows for quantitative analysis of CO2.

This report introduces a case where CO2 generated from commercially available cement was quantified when heated under a simulated air atmosphere (He/O2=4/1 (v/v)). **[Analysis Sample]** Commercial powder cement **[Analysis Equipment]** TG-DTA: Rigaku Thermo plus EVO2 GC-MS: Agilent 8890GC/5977B Inert Plus **[Analysis Method and Results]** CO2 generated from the sample was quantified using the TG-MS method while heating the sample from around 30°C to 1,000°C (20°C/min) under a simulated air atmosphere. After creating a calibration curve (Figure 1) using standard substances, a SIM ion thermogram was drawn using the characteristic ion of CO2 generated from the sample (m/z 44), and the amount of CO2 generated in the temperature range of 513 to 825°C was determined. The results are shown in Table 1. As a result, it was found that the sample measured generated 30 wt% of CO2. **[Summary]** The TG-MS method allows for the quantification of CO2 generated from the sample over any temperature range. Additionally, since the measurement atmosphere can be chosen from inert gas (He) and simulated air, analysis that simulates the usage environment is possible.

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[Analysis Case] Analysis of the Thermal Decomposition Behavior of Polyimide (TG-MS)

[Analysis Case] Analysis of the Thermal Decomposition Behavior of Polyimide (TG-MS)

I will introduce a case that analyzed the thermal decomposition behavior of polyimide.

The TG-MS (Thermogravimetric-Mass Spectrometry) method introduces gases generated during the TG-DTA (Thermogravimetric Differential Thermal Analysis) measurement of a sample online to an MS (Mass Spectrometer) for analysis. This technique allows for the acquisition of information regarding the thermal decomposition behavior of the sample, the types of gases generated, and the temperature ranges at which these gases are produced. Additionally, by using the sample observation function, changes in the shape and color of the sample during heating can be observed in real time. **[Analyzed Sample]** Polyimide (PI) film **[Analytical Equipment]** TG-DTA: Rigaku Thermo plus EVO2 GC-MS: Agilent 8890GC/5977B Inert Plus **[Analysis Results]** A weight loss of the PI film was observed around 540°C, accompanied by the detection of CO, CO2, and decomposition components such as Phenol and Benzonitrile. Furthermore, the sample observation images showed that the PI film gradually changed from orange to brown and then to black, and shrinkage was also observed starting around 522°C. **[Summary]** The TG-MS method allows for understanding the types of gases generated and the weight loss behavior of the sample during heating.

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